BioMed Research International / 2015 / Article / Fig 4

Research Article

Potentiated Osteoinductivity via Cotransfection with BMP-2 and VEGF Genes in Microencapsulated C2C12 Cells

Figure 4

Assessment of potentiated osteoinductivity. (a) Measurement of cytokine expressions after the cotransfection of BMP-2 and VEGF in microencapsulated C2C12 cells. After the cotransfection of BMP-2 and VEGF in C2C12 cells, the microencapsulated C2C12 cells could release both BMP-2 and VEGF over a span of four weeks ( compared with the 1-week observation time point). (b) The ALP activity of BMSCs cocultured with the microencapsulated C2C12 cells. BMP2 + VEGF: cocultured with the microencapsulated BMP-2 and VEGF gene-transfected C2C12 cells. BMP2: cocultured with the microencapsulated BMP-2 gene-transfected C2C12 cells. Control: coculture with the microencapsulated C2C12 lacking gene transfection (). (c) The ALP staining of BMSCs cocultured with microencapsulated C2C12 cells. (A) showed the staining results after 3 days of coculture. (B) showed the staining results after 7 days of coculture. (1) Cocultured with the microencapsulated BMP-2 and VEGF gene-transfected C2C12 cells. (2) Cocultured with the microencapsulated BMP-2 gene-transfected C2C12 cells. (3) Cocultured with the microencapsulated none gene-transfected C2C12 cells. (d) The Alizarin Red Staining of BMSCs cocultured with the microencapsulated C2C12 cells. (A) Cocultured with the microencapsulated BMP-2 and VEGF gene-transfected C2C12 cells. (B) Cocultured with the microencapsulated BMP-2 gene-transfected C2C12 cells. (C) Cocultured with the microencapsulated C2C12 cells lacking gene transfection.
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