Experimental Protoporphyria: Effect of Bile Acids on Liver Damage Induced by Griseofulvin

Figure 1

Effect of bile acids on liver histology and HO1 immunostaining. (a) Gris (0.5% w/w) histology showing: liver plates disrupted and distorted; hepatocellular degeneration with small foci of necrosis (circles); ductular proliferation; portal chronic infiltrate (arrows) and cytoplasmic and canalicular cholestasis. HO1 immunostaining: (b) Gris (0.5% w/w) (arrows); (c) Gris (0.5% w/w) plus DHA (0.33% w/v) (arrows); (d) Gris (0.5% w/w) plus CHENO (0.01% w/w) (arrows); (e) Gris (0.5% w/w) plus DXA (0.33% w/w) positive isolated hepatocytes and Kupffer’s cells (arrows); (f) Gris (0.5% w/w) plus URSO (0.2% w/w) positive peri centrilobular vein hepatocytes (circle) and Kupffer’s cells (arrows); (g) control; (h) control HO1 immunostaining. A unique photo of controls was shown because there were no differences between this group and the groups only treated with the bile acids. Experimental details are described in Section 2.

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