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BioMed Research International
Volume 2015, Article ID 437328, 11 pages
http://dx.doi.org/10.1155/2015/437328
Research Article

Transcriptional and Biochemical Effects of Cadmium and Manganese on the Defense System of Octopus vulgaris Paralarvae

1Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Coastal Marine Environment, UOS Capo Granitola, Torretta Granitola, 91021 Trapani, Italy
2National Research Council, Institute for Coastal Marine Environment, Calata Porta di Massa, 80133 Naples, Italy

Received 2 September 2014; Revised 6 November 2014; Accepted 27 November 2014

Academic Editor: Francesco Dondero

Copyright © 2015 Aldo Nicosia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Due to anthropogenic activities the relative concentrations of cadmium and manganese have increased in the marine environment. Cephalopods are able to accumulate such metals and, as inhabitant of coastal waters, Octopus vulgaris is continuously exposed to anthropogenic activities. Since no study is available on the effects of heavy metals at molecular level in developing octopuses, herein we exposed 1-day-old paralarvae for 24 h to 10, 100, and 1000 μg/L of CdCl2 or MnCl2. Cd exerted a concentration-dependent inhibition of survival and a reduction in growth rate was shown while Mn exposure did not affect the survival rate even at the highest concentrations. Gene expression profiles of hsp70, sod, cat, and gst genes were analyzed by quantitative real-time PCR and defined patterns of transcription were observed. Moreover posttranscriptional analyses were also performed suggesting the impairment of metabolic functions, under strong oxidative conditions (as occurred in paralarvae exposed to Cd) or the complete detoxification events (as occurred in paralarvae exposed to Mn).