Research Article

Transcriptional and Biochemical Effects of Cadmium and Manganese on the Defense System of Octopus vulgaris Paralarvae

Figure 3

Effects of cadmium (a) and manganese (b) exposure on expression of HSP70 heat shock protein in O. vulgaris paralarvae. Representative immunoblot of HSP70 in paralarvae collected 24 h after exposure to CdCl2 and MnCl2 at the indicated concentrations. The histograms represent the average fold increase values ± SD of HSP70 calculated, after normalization with actin levels. Asterisks indicate statistically significant differences versus nonexposed controls at .