Research Article

Estrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast

Table 4

Concentrations (ng/mL) of 13 organochlorine compounds in the extracts of 12 samples of North Sea harbour porpoise blubber, in culture medium solution to which the ER sensitive cells were exposed in the cell based assay (calculated from GC-MS data from Table 3; according to that 1 mL of culture medium contains the contaminants extracted from 0.04 g of fat). For the samples displaying an estrogenic activity, the percentage of relative response compared to the maximal response induced by E2 is indicated between brackets.

03/152101/119603/1238A00/1140A00/258A03/151701/84701/80501/1219A00/60001/887A00/974

PCB 1384.0 × 1015.2 × 1013.3 × 1021.5 × 1011.5 × 1021.3 × 1031.1 × 1021.5 × 1023.1 × 1021.4 × 1029.1 × 1012.4 × 101
PCB 1535.4 × 1016.7 × 1014.9 × 1022.5 × 1022.2 × 1021.7 × 1031.6 × 1022.2 × 1024.5 × 1022.1 × 1021.3 × 1022.4 × 101
PCB 1801.3 × 1011.3 × 1011.0 × 1027.3 × 1014.7 × 1013.0 × 1023.2 × 1014.9 × 1017.5 × 1013.7 × 1013.1 × 1014.5 × 100
Σ (3) PCBs1.1 × 1021.3 × 1029.2 × 1024.8 × 1024.2 × 1023.2 × 1033.0 × 1024.2 × 1028.4 × 1023.9 × 1022.5 × 1025.2 × 101

o,-DDT1.9 × 1004.2 × 1001.4 × 1013.9 × 1003.0 × 1008.5 × 1002.4 × 1003.3 × 1003.4 × 1012.2 × 1006.7 × 1002.8 × 100
o,-DDDn/a2.9 × 1007.2 × 1003.1 × 1002.7 × 1001.7 × 101n/a3.4 × 1002.1 × 101n/a2.8 × 1001.6 × 100
p,-DDT7.2 × 1001.3 × 1013.2 × 1012.2 × 1011.9 × 1012.8 × 1017.5 × 1001.5 × 1015.6 × 1018.0 × 1002.3 × 1014.3 × 100
p,-DDD1.4 × 1012.1 × 1013.5 × 1013.7 × 1013.4 × 1015.0 × 1019.5 × 1002.9 × 1011.0 × 1021.6 × 1012.4 × 1015.6 × 100
p,-DDE4.2 × 1015.0 × 1011.2 × 1021.1 × 1021.2 × 1021.6 × 1023.6 × 1019.6 × 1013.4 × 1025.6 × 1019.3 × 1012.0 × 101
(5) DDTs6.4 × 1019.1 × 1012.1 × 1021.7 × 1021.8 × 1022.7 × 1025.6 × 1011.5 × 1025.6 × 1028.2 × 1011.5 × 1023.5 × 101

-HCHn/an/a3.9 × 100n/a3.5 × 1003.2 × 100n/a3.3 × 1005.0 × 1001.7 × 1003.0 × 1001.9 × 100
-HCHn/an/an/an/an/an/an/an/an/an/an/an/a
-HCH3.9 × 1005.8 × 1005.3 × 1005.1 × 1009.4 × 1007.8 × 1003.2 × 1006.9 × 1001.9 × 1017.0 × 1006.1 × 1004.6 × 100
-HCH2.8 × 100n/a2.7 × 1002.6 × 100n/an/an/a3.2 × 1003.9 × 1002.8 × 1004.2 × 1003.0 × 100
HCB5.7 × 1008.4 × 1002.1 × 1018.9 × 1001.2 × 1012.0 × 1015.4 × 1001.3 × 1016.1 × 1013.9 × 1002.4 × 1013.0 × 100
(5) HCHs + HCB1.2 × 1011.4 × 1013.3 × 1011.7 × 1012.5 × 1013.1 × 1018.6 × 1002.7 × 1018.9 × 1011.5 × 1013.7 × 1011.3 × 101

(13) total1.9 × 1022.4 × 1021.2 × 1036.7 × 1026.2 × 1023.5 × 1033.7 × 1025.9 × 1021.5 × 1034.8 × 1024.4 × 1029.9 × 101

Activity recorded in the ER reporter gene assay (% of relative response)NoneAntiestrogenic (7%)Antiestrogenic (16%)Antiestrogenic (6%)Estrogenic (22%)Antiestrogenic (13%)Antiestrogenic (5%)NoneEstrogenic (24%) and antiestrogenic (6%)Antiestrogenic (5%)None Antiestrogenic (7%)

n/a: not applicable.