Revealing Glycoproteins in the Secretome of MCF-7 Human Breast Cancer Cells

Figure 2

Typical representative of O-glycoprotein profile of MCF-7 growth medium. Harvested growth media were subjected to 2D-E, blotted, and then developed with HRP-conjugated CGB lectin. Protein spots of HSC70 and CPA4 were detected. No O-glycoproteins were detected in HMEpC growth medium (not shown).