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BioMed Research International
Volume 2015 (2015), Article ID 453289, 8 pages
http://dx.doi.org/10.1155/2015/453289
Research Article

Revealing Glycoproteins in the Secretome of MCF-7 Human Breast Cancer Cells

1Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, Malaysia
2Department of Oral Biology & Biomedical Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia
3Institute of Nano Electronic Engineering (INEE) and School of Bioprocess Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia
4Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
5Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
6Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia

Received 13 April 2015; Accepted 7 June 2015

Academic Editor: Marko Pesu

Copyright © 2015 Aik-Aun Tan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Shown are the mass spectra of carboxypeptidase A4 (a), alpha-1-antitrypsin (b), haptoglobin (c), HSC-70 (d), and osteonectin (e). The protein spots were excised from the 2-DE gel, in-gel trypsin digested, ZipTip purified, and were analyzed with MALDI-TOF/TOF MS. Peaks of the tryptic peptides are shown in the spectrum.

  1. Supplementary Material