miR-136 Modulates TGF-β1-Induced Proliferation Arrest by Targeting PPP2R2A in Keratinocytes

Table 1

Oligonucleotides for dual-luciferase reporter assays. All oligonucleotides for 3 target sites were designed with proper overhangs for SacI and XbaI digestion. Mutation sites were shown in italics and KpnI restriction enzyme sites were shown in bold.

Name	Oligonucleotides sequence
wt site 1, sense	5^′-CGGTACCCAACACATTGTTATAGCTACATGGAGAAAGCTT-3^′
wt site 1, antisense	5^′-CTAGAAGCTTTCTCCATGTAGCTATAACAATGTGTTGGGTACCGAGCT-3^′
mut site 1, sense	5^′-CGGTACCCAACACATTGTTATAGCTACGGAACTAAAGCTT-3^′
mut site 1, antisense	5^′-CTAGAAGCTTTAGTTCCGTAGCTATAACAATGTGTTGGGTACCGAGCT-3^′
wt site 2, sense	5^′-CGGTACCACTGAATAAAAGTGACAAAGAATGGAGAATCTGT-3^′
wt site 2, antisense	5^′-CTAGACAGATTCTCCATTCTTTGTCACTTTTATTCAGTGGTACCGAGCT-3^′
mut site 2, sense	5^′-CGGTACCACTGAATAAAAGTGACAAAGCGGAACTAATCTGT-3^′
mut site 2, antisense	5^′-CTAGACAGATTAGTTCCGCTTTGTCACTTTTATTCAGTGGTACCGAGCT-3^′
wt site 3, sense	5^′-CGGTACCGTCAGTCTTGCAGAAACTTTAATGGAGAAGAAAT-3^′
wt site 3, antisense	5^′-CTAGATTTCTTCTCCATTAAAGTTTCTGCAAGACTGACGGTACCGAGCT-3^′
mut site 3, sense	5^′-CGGTACCGTCAGTCTTGCAGAAACTTTCGGAACTAAGAAAT-3^′
mut site 3, antisense	5^′-CTAGATTTCTTAGTTCCGAAAGTTTCTGCAAGACTGACGGTACCGAGCT-3^′