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BioMed Research International
Volume 2015, Article ID 453748, 8 pages
http://dx.doi.org/10.1155/2015/453748
Research Article

The Effect of Iloprost and N-Acetylcysteine on Skeletal Muscle Injury in an Acute Aortic Ischemia-Reperfusion Model: An Experimental Study

1Department of Cardiovascular Surgery, Bahcesehir University Medical Faculty, Istanbul, Turkey
2Department of Cardiovascular Surgery, Medical Park Bursa Hospital, Bursa, Turkey
3Department of Cardiovascular Surgery, Osmangazi University Medical Faculty, Eskisehir, Turkey
4Vocational School of Health Services, Osmangazi University Medical Faculty, Eskisehir, Turkey
5Department of Cardiovascular Surgery, Mehmet Akif Ersoy GKDCEA Hospital, Istanbul, Turkey
6Department of Cardiovascular Surgery, Pamukkale University Medical Faculty, Denizli, Turkey
7Department of Cardiovascular Surgery, Acibadem Atakent Hospital, Istanbul, Turkey

Received 6 December 2014; Revised 1 February 2015; Accepted 13 February 2015

Academic Editor: Kazim Husain

Copyright © 2015 Osman Tiryakioglu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. The objective of this study was to examine the effects of iloprost and N-acetylcysteine (NAC) on ischemia-reperfusion (IR) injuries to the gastrocnemius muscle, following the occlusion-reperfusion period in the abdominal aorta of rats. Materials and Methods. Forty male Sprague-Dawley rats were randomly divided into four equal groups. Group 1: control group. Group 2 (IR): aorta was occluded. The clamp was removed after 1 hour of ischemia. Blood samples and muscle tissue specimens were collected following a 2-hour reperfusion period. Group 3 (IR + iloprost): during a 1-hour ischemia period, iloprost infusion was initiated from the jugular catheter. During a 2-hour reperfusion period, the iloprost infusion continued. Group 4 (IR + NAC): similar to the iloprost group. Findings. The mean total oxidant status, CK, and LDH levels were highest in Group 2 and lowest in Group 1. The levels of these parameters in Group 3 and Group 4 were lower compared to Group 2 and higher compared to Group 1 (). The histopathological examination showed that Group 3 and Group 4, compared to Group 2, had preserved appearance with respect to hemorrhage, necrosis, loss of nuclei, infiltration, and similar parameters. Conclusion. Iloprost and NAC are effective against ischemia-reperfusion injury and decrease ischemia-related tissue injury.