Identification and Validation of Potential New Biomarkers for Prostate Cancer Diagnosis and Prognosis Using 2D-DIGE and MS

Figure 4

Western blot analysis of secernin-1 (SCRN1) and -actin as a house keeping protein in prostate cancer tissue and tumor-free tissue samples. (a) Western blot analysis. (b) Relative SCRN1 expression levels were calculated densitometrically in reference to the β-actin expression level. (c) Boxplot of the densitometrically determined SCRN1 expression levels. A significant difference between tumors without ( − rec) and with recurrence ( + Rec) is not detectable () but SCRN1 is significantly downregulated in prostate cancer tissue compared to tumor-free tissue samples (tf) ().