Activation of Endocannabinoid System Is Associated with Persistent Inflammation in Human Aortic Aneurysm

Figure 4

Collagen area and expression of remodeling related factors in aortic aneurysm. Representative images of picrosirius red staining show comparable collagen-deposition pattern in media (m) and adventitia (a) of (a) aorta from patient with coronary artery disease (CAD) and (b) aneurysm of the ascending aorta (AAA). (c) The planimetric evaluation of the collagen-stained area in the inner and outer media showed comparable results between both groups. The mRNA expression of (d) the collagen-related connective tissue growth factor (CTGF) and (e) extracellular matrix remodeling related factor osteopontin-1 is significantly lower in AAA when compared to the CAD aortic samples. (f) The mRNA expressions of early remodeling factor tenascin C and (g) transforming growth factor- (TGF-) β1 are comparable between the groups. in the AAA group, in the CAD histology, and in the CAD RT-qPCR group. mRNA expression in RT-qPCR is related to controls and GAPDH using comparative ΔΔCt method. Bracket indicates .

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