Cholesteryl Pullulan Encapsulated TNF-α Nanoparticles Are an Effective Mucosal Vaccine Adjuvant against Influenza Virus

Table 1

Primer sets for quantitative PCR.

Name	Genbank; accession number	Forward	Reverse	Length/Tm; (bp/°C)
IFN-	NM_008337	tcaagtggcatagatgtggaagaa	tggctctgcaggattttcatg	92/60
IL-4	NM_009841	acaggagaagggacgccat	gaagccctacagacgagctca	95/60
IL-1	NM_010554	atgtatgcctactcgtcggg	tgagttttggtgtttctggc	139/60
IL-1	NM_008361	caaccaacaagtgatattctccatg	gatccacactctccagctgca	152/60
IL-2	NM_008366	cctgagcaggatggagaattaca	tccagaacatgccgcagag	141/60
IL-5	NM_010558	agcacagtggtgaaagagacctt	tccaatgcatagctggtgattt	117/60
IL-6	NM_031168	agttgccttcttgggactga	ttgccattgcacaactcttt	186/60
IL-10	NM_010548	ggttgccaagccttatcgga	acctgctccactgccttgct	191/62
IL-12	NM_008352	agctcgcagcaaagcaaggt	tggagacaccagcaaaacga	181/62
IL-17A	NM_010552	gctccagaaggccctcaga	agctttccctccgcattga	142/60
IL-18	NM_008360	ccaaatcacttcctcttggc	ggccaaagttgtctgattcc	144/60
IFN-1	NM_010510	ccgagcagagatcttcaggaa	cctgcaaccaccactcattct	106/60
fibronectin 1	NM_010233	gggagaagtttgtgcatggt	ctgggggtctccgtgataat	136/60
CD14	NM_009841	catttgcatcctcctggtttctga	gagtgagttttccccttccgtgtg	182/55
proteoglycan 2	NM_008920	acttgacaagacccaggagg	ctcatccatcaatgggcttt	135/60
LPS binding protein	NM_008489	tcgccatctctgactcttcc	ggaggtccactgaaatggtg	120/60
Lactotransferrin	NM_008522	aatccaatctctgtgccctg	atgcaacatttcctgccttc	132/60
TLR3	NM_126166	tgcagtctttccagagggat	acaaaagtcccccaaaggag	133/60
CXCL2	NM_009140	gagcttgagtgtgacgcccccagg	gttagccttgcctttgttcagtatc	148/58
TNF-	NM_013693	cgtcgtagcaaaccaccaag	gtgggtgaggagcacgtagt	187/62
GAPDH	NM_002046	accatcttccaggagcgag	agtgatggcatggactgtgg	324/60