Melanoma Cells Homing to the Brain: An In Vitro Model
(a) Transmigration of M1 and M2 cells across the HUVECs monolayer cocultered with astrocytes (in vitro BBB). 100.000 cells were added in the upper chamber and, after 24 hours, transmigrated cells were counted in the lower chamber (see Material end Methods). Transmigrated cells are expressed as percentage of the total number of melanoma cells used for each insert. Data are expressed as mean ± SD (error bars) from at least six independent experiments. M2 cells showed a statistically significant increased migration compared to M1 (, t-test). (b) Transmigration ability through the polycarbonate membrane alone was similar in M1 and M2 cells. 100.000 cells were loaded in the upper well of the Transwell chamber and incubated for 24 h. Data are expressed as mean percentage of the total number of cells ± SD (error bars) from at least six independent experiments.