Melanoma Cells Homing to the Brain: An In Vitro Model

Figure 5

To exclude major differences in cell volume between M1 and M2 cells eventually accounting for the different ability to transmigrate, the forward scatter of M1 (black bar) and M2 (grey bar) cell was measured by flow cytometry. Despite M2 showing a statistically significant increased migration through the in vitro BBB compared to M1, their volume was slightly higher than that of M1 (310 versus 250 arbitrary unit) suggesting that cell volume was not responsible for the differences observed in transmigration.