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BioMed Research International
Volume 2015, Article ID 478017, 6 pages
http://dx.doi.org/10.1155/2015/478017
Research Article

Towards a Next-Generation Sequencing Diagnostic Service for Tumour Genotyping: A Comparison of Panels and Platforms

1Yorkshire Regional Genetics Service, St. James’s University Hospital, Leeds LS9 7TF, UK
2Leeds Institute of Cancer & Pathology, University of Leeds, St. James’s University Hospital, Leeds LS9 7TF, UK
3Leeds Institute of Biomedical & Clinical Sciences, University of Leeds, St. James’s University Hospital, Leeds LS9 7TF, UK

Received 8 June 2015; Accepted 10 August 2015

Academic Editor: Pankaj Kumar

Copyright © 2015 George J. Burghel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary material table 1 provides the details of the 8 cell lines mutation status, supplementary material tables 2-4 list the target regions/genes included within the three different target enrichment panels; Fluidigm Access Array custom amplicon panel, the Ion AmpliSeq Cancer Hotspot Panel and the OGT SureSeq Solid Tumour hybridisation panel. Supplementary tables 5-7 provide the per-sample read distribution of the three different target enrichment panels. Supplementary table 8 shows the depth of coverage and mutant allele frequency of all of the variants detected in the cell lines’ fresh gDNA samples using the Fluidigm custom panel and the OGT SureSeq panel. Supplementary table 9 shows the depth of coverage and mutant allele frequency of all of the variants detected in the cell lines’ FFPE gDNA samples using the three target enrichment panels. Supplementary figure S1 shows the Ion PGM sequencing output information.

  1. Supplementary Material