Figure 5: DNA ladder formation shown by gel electrophoresis. DNA was prepared from dissociated cortical cells from neonatal rats (P14) exposed to 0.5 pM, 50 nM, and 5 μM kainic acid (KA) for 180 min, as well as from a control group. The DNA mixture was separated by 1.5% agarose gel. Lane 1 shows no DNA fragments in the cells from untreated dissociated cortical cells. Lanes 2 and 3 show no DNA fragments in the dissociated cortical cells treated with 5 μM and 50 nM KA for 180 min. Lane 4 shows DNA fragments from dissociated cortical cells treated with 0.5 pM KA for 180 min.