Key Proteins of Activating Cell Death Can Be Predicted through a Kainic Acid-Induced Excitotoxic Stress

Figure 7

Comparison of electrophoretic protein profiles from apoptotic and necrotic cells. Following 180 min of treatment with 0.5 pM and 5 μM kainic acid (KA), each dissociated cortical neuron cell lysate was separated into three different two-dimensional gel electrophoresis (2-DE) runs and stained with silver-stain. Figures are from representative gels demonstrating typical protein profiles of 5 μM induced necrotic cells (a) and 0.5 pM KA-induced apoptotic cells (b). More than 1500 protein spots were routinely detected. The red circles indicate protein spots that were altered significantly by at least 1.5-fold with a resulting increase in apoptotic cells compared with necrotic cells based on data from at least three independent gels ().

(a)

(b)