Ouabain-Induced Cytoplasmic Vesicles and Their Role in Cell Volume Maintenance

Figure 2

Comparison at low magnification between slices after 60 min recovery at 38°C in the presence (b) or absence (a) of 2 mM ouabain. (a) Control liver slices after incubation for 90 min at 1°C followed by 60 min at 38°C. The ultrastructure has recovered well from the alterations in the cold preincubation (Figure 1) (see [6]). The cytosol and organelles appear to have a normal electron density. (b) Liver slice after 90 min at 1°C followed by 60 min at 37°C in the presence of 2 mM ouabain. A large number of rounded, clear vesicles are present, mainly localized in the Golgi region towards and around the bile canaliculi. The ground substance and other subcellular components are similar to the control in (a).

(a)

(b)