Ouabain-Induced Cytoplasmic Vesicles and Their Role in Cell Volume Maintenance

Figure 3

Liver slices were incubated at 1°C for 90 min and then at 38°C for 15 min in a Cl⁻ free medium ( or ) and, finally, were transferred in a Cl⁻ containing medium at 38°C for 60 min. (a) Water transport in the presence of ouabain was efficiently prevented by replacement of Cl⁻ in the medium with (upper panel) or with (lower panel). (b) At the end of incubation in Cl⁻ free medium in the presence of ouabain, the almost complete absence of typical vesicles was evident. (c) Transferring slices from Cl⁻ free medium to normal medium produces the appearance of ouabain-induced vesicles. BC = bile canaliculus.