Ouabain-Induced Cytoplasmic Vesicles and Their Role in Cell Volume Maintenance

Figure 8

Liver slices were incubated for 90 min at 1°C followed by 60 min at 38°C, with 10 μM phalloidin plus 2 mM ouabain [7]. (a) This micrograph shows an increase in the size and number of vesicles. These show a rather irregular outline probably resulting from the fusion of smaller vesicles. The vesicular contents are clear and similar to that seen with ouabain alone. (b) Detail of a very large vesicle close to a small bile canaliculus. (c) Detail of homogeneous submembrane zone typical of the stabilization effect of phalloidin on cortical actin. BC = bile canaliculus; V = vesicles; S = sinusoid.