Ouabain-Induced Cytoplasmic Vesicles and Their Role in Cell Volume Maintenance

Table 1

Time-course of (a) water and (b) ionic content of rat liver slices during incubation at 38°C, in the presence or absence of ouabain [6], after 90 min incubation at 1°C. Points at zero time represent the tissue composition at the end of cold incubation at 1°C [8].

Treatment	Time at 38°C (min)	Water (kg/kg dry weight)			Ions (mmol/kg dry weight)
		Total	Intracellular	Extracellular	Na^+	Cl^−	K^+
Control	0	3.5 ± 0.1	2.7 ± 0.2	1.0 ± 0.1	350 ± 30	320 ± 20	75 ± 10
	15	2.7 ± 0.2	1.6 ± 0.3	1.2 ± 0.1	180 ± 20	175 ± 35	90 ± 12
	60	2.7 ± 0.2	1.5 ± 0.2	1.3 ± 0.1	100 ± 15	180 ± 25	150 ± 15
Ouabain 2 mM	0	3.4 ± 0.2	2.7 ± 0.2	0.9 ± 0.1	360 ± 25	335 ± 20	70 + 10
	15	3.1 ± 0.5	2.2 ± 0.2	1.0 ± 0.2	375 ± 30	350 ± 15	62 + 15
	60	3.1 ± 0.6	2.2 ± 0.4	1.0 ± 0.2	420 ± 10	380 ± 30	50 + 10