{2-[1-(3-Methoxycarbonylmethyl-1H-indol-2-yl)-1-methyl-ethyl]-1H-indol-3-yl}-acetic Acid Methyl Ester Inhibited Hepatocellular Carcinoma Growth in Bel-7402 Cells and Its Resistant Variants by Activation of NOX4 and SIRT3

Figure 2

MIAM induced apoptosis in HCC cells. (a-b) Cells were exposed to different concentrations of MIAM for 72 h and then the percent of apoptotic cells were determined by using flow cytometry assay. (c) MIAM induced mitochondrial membrane potential collapse in Bel-7402 and Bel-7402/5FU cells as observed by JC-1 staining. The bars indicate mean ± S.D. (). , versus the vehicle control. (d) MIAM regulated Bax and Bcl-2 expressions in Bel-7402 and Bel-7402/5FU cells. Data are representative of 3 independent experiments.

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