{2-[1-(3-Methoxycarbonylmethyl-1H-indol-2-yl)-1-methyl-ethyl]-1H-indol-3-yl}-acetic Acid Methyl Ester Inhibited Hepatocellular Carcinoma Growth in Bel-7402 Cells and Its Resistant Variants by Activation of NOX4 and SIRT3

Figure 4

MIAM increased the level of cellular ROS in HCC cells. (a-b) Bel-7402 and Bel-7402/5FU cells were exposed to MIAM and ADR for 72 h, respectively. After staining with DCFH-DA probe, the levels of cellular ROS were visualized by ArrayScan XTI high-content imaging instrumentation. (c) Western blotting analyzed the expression of NOX4, , and BIM in HCC cells. Data are representative of 3 independent experiments.

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