A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome

Figure 4

Effect of SSV mAb on histopathology of lung tissue. Lung tissue specimens were obtained 12 hr after LPS injection. The specimens were inflated and fixed in 10% formaldehyde and then subjected to a paraffin blocking procedure. Cross-sections were stained with hematoxylin-eosin and observed under a microscope at 40x magnification. Bars represent 500 μm in all panels. (a) A representative sample from a normal mouse that did not receive LPS injection. (b) A representative sample from a mouse that received LPS injection and was treated with a control (anti-mouse IgG1) antibody. (c) A representative sample from a mouse that received LPS injection and was treated with SSV mAb. (d) Images represented by those in panels b and c were divided into 24 separate areas and scored on a 0–4 scale for the level of change (from normal mice) in congestion, edema, inflammation, and hemorrhage. Values presented are means ± standard deviation. ^*Significant differences ().

(a) Normal

(b) Control group

(c) SSV mAb group

(d)