Disturbance of Oligodendrocyte Function Plays a Key Role in the Pathogenesis of Schizophrenia and Major Depressive Disorder

Figure 6

DBZ is transiently upregulated in vitro during oligodendrocyte differentiation before myelin marker expression. (a) Primary OPC were isolated from rat postnatal cortex and differentiated by mitogen withdrawal. Total RNA was prepared from the cells 0, 2, 4, 6, or 8 days after mitogen withdrawal. DBZ mRNA expression was evaluated using qRT-PCR, with normalization to GAPDH. Data normalized to either day 0. , versus day 0 or +, and ++ versus day 2 by Dunnett’s test following one-way ANOVA, –4. (b) Western blot analyses of DBZ, MBP and CNPase from cells 0, 2, 4, 6, or 8 days after mitogen withdrawal. (a) and (b) are adapted with permission from Shimizu et al. [19].

(a)

(b)