miR-126 Is Involved in Vascular Remodeling under Laminar Shear Stress

Figure 4

Study of HUVEC transfection after 48 h with scramble, anti-miR-126, and pre-miR-126 under static conditions. (a) Confirmation of miR-126 overexpression and knockdown under the respective conditions. Values are expressed as mean ± SD of 3 independent experiments (AU: arbitrary units). Student’s -test, for miR-126: (scramble versus anti-miR-126), (scramble versus pre-miR-126). (b) HUVEC metabolic activity was measured using MTT test after HUVEC transfection. Representative flow cytometry histogram study of apoptosis from HUVECs. (c) Expression of various mRNA targets in HUVECs after 48 h of transfection with anti-miR-126 and pre-miR-126. Values are expressed as mean ± SD of 3 independent experiments (AU: arbitrary units). Student’s -test, for VCAM-1: (scramble versus anti-miR-126), (scramble versus pre-miR-126); for RGS-16, SDC-4, CXCR4: (scramble versus anti-miR-126), (scramble versus pre-miR-126); for CXCL12: (scramble versus anti-miR-126), (scramble versus pre-miR-126).

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