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BioMed Research International
Volume 2015 (2015), Article ID 497462, 8 pages
http://dx.doi.org/10.1155/2015/497462
Research Article

Optimization of Fermentation Medium for Extracellular Lipase Production from Aspergillus niger Using Response Surface Methodology

1Shenzhen Leveking Bio-Engineering Co., Ltd., Guangdong, Shenzhen 518055, China
2School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong
3Shenzhen Institute of Technology, Guangdong, Shenzhen 518116, China

Received 31 May 2015; Revised 8 August 2015; Accepted 10 August 2015

Academic Editor: Denise Freire

Copyright © 2015 Jia Jia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Lipase produced by Aspergillus niger is widely used in various industries. In this study, extracellular lipase production from an industrial producing strain of A. niger was improved by medium optimization. The secondary carbon source, nitrogen source, and lipid were found to be the three most influential factors for lipase production by single-factor experiments. According to the statistical approach, the optimum values of three most influential parameters were determined: 10.5 g/L corn starch, 35.4 g/L soybean meal, and 10.9 g/L soybean oil. Using this optimum medium, the best lipase activity was obtained at 2,171 U/mL, which was 16.4% higher than using the initial medium. All these results confirmed the validity of the model. Furthermore, results of the Box-Behnken Design and quadratic models analysis indicated that the carbon to nitrogen (C/N) ratio significantly influenced the enzyme production, which also suggested that more attention should be paid to the C/N ratio for the optimization of enzyme production.