Optimization of Fermentation Medium for Extracellular Lipase Production from Aspergillus niger Using Response Surface Methodology

Table 4

Validation experiments of the optimum medium ().

Medium	Lipase activity (U/mL)
	Batch 1	Batch 2	Batch 3	Mean ± SD	CV (%)
FM1	1863 ± 27	1835 ± 30	1896 ± 62	1865 ± 45	2.18
FM2	2046 ± 34	1996 ± 63	2030 ± 59	2024 ± 52	2.27
FM3	2174 ± 46	2161 ± 54	2177 ± 39	2171 ± 41	1.7

Lipase activity in FM2 and FM3 increased by 8.5% and 16.4%, respectively, compared to FM1.