Aberrant Hypermethylation of Aldehyde Dehydrogenase 2 Promoter Upstream Sequence in Rats with Experimental Myocardial Infarction

Table 2

Primer sequences, product length, and CpG units used for MassARRAY.

Primer; number	Forward primer (5^′-3^′)^1	Reverse primer (5^′-3^′)^2	Product; length (bp)	CpG unit
SQ1	TTAAGGATTTGTTTGTATTTAATTGG	CAAACAATACCACAATTTATATCTTTTA	416	6
SQ2	AGATTTGGAGAGATGGTTTAGTGGT	ACCCATTTCCTACAAAAAAATATCC	498	10
SQ3	TTAAAAGATATAAATTGGGGTTGGG	ACCAACAAAACCCCTCTAAATAAAC	349	9

For 10-mer tag, CAGTAATACGACTCACTATAGGGAGAAGG was added and for T7 promoter tag AGGAAGAGAG was added.