Aberrant Hypermethylation of Aldehyde Dehydrogenase 2 Promoter Upstream Sequence in Rats with Experimental Myocardial Infarction

Table 4

BSP analysis of DNA methylation level of CpG sites in ALDH2 core promoter.

(a) M1

CpG number	1	2	3	4	5	6	7	8	9
A1	0	0	0	0	0	0	0	0	0
A2	0	0	0	0	0	0	0	0	0
A3	0	0	0	0	0	0	0	0	0
A4	0	0	0	0	0	0	0	0	0
A5	0	0	0	0	0	0	0	0	0
B1	0	0	0	0	0	0	0	0	0
B2	0	0	0	0	0	0	0	0	0
B3	0	0	0	0	0	0	0	0	0
B4	0	0	0	0	0	0	0	0	0
B5	0	0	0	0	0	0	0	0	0
C1	0	0	0	0	0	0	0	0	0
C2	0	0	0	0	0	0	0	0	0
C3	0	0	0	0	0	0	0	0	0
C4	0	0	0	0	0	0	0	0	0
C5	0	0	0	0	0	0	0	0	0
Sh1	0	0	0	0	0	0	0	0	0
Sh2	0	0	0	0	0	0	0	0	0
Sh3	0	0	0	0	0	0	0	0	0
Sh4	0	0	0	0	0	0	0	0	0
Sh5	0	0	0	0	0	0	0	0	0

(b) M2

CpG number	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19
A1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
A2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
A3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
A4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
A5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
B1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
B2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
B3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
B4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
B5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
C1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
C2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
C3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
C4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
C5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sh1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sh2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sh3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sh4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sh5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

(c) M3

CpG number	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20
A1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
A2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
A3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
A4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
A5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
B1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
B2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
B3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
B4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
B5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
C1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
C2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
C3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
C4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
C5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sh1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sh2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sh3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sh4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Sh5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

(d) M4

CpG number	1	2	3	4	5	6	7	8	9	10
A1	0	0	0	0	0	0	0	0	0	0
A2	0	0	0	0	0	0	0	0	0	0
A3	0	0	0	0	0	0	0	0	0	0
A4	0	0	0	0	0	0	0	0	0	0
A5	0	0	0	0	0	0	0	0	0	0
B1	0	0	0	0	0	0	0	0	0	0
B2	0	0	0	0	0	0	0	0	0	0
B3	0	0	0	0	0	0	0	0	0	0
B4	0	0	0	0	0	0	0	0	0	0
B5	0	0	0	0	0	0	0	0	0	0
C1	0	0	0	0	0	0	0	0	0	0
C2	0	0	0	0	0	0	0	0	0	0
C3	0	0	0	0	0	0	0	0	0	0
C4	0	0	0	0	0	0	0	0	0	0
C5	0	0	0	0	0	0	0	0	0	0
Sh1	0	0	0	0	0	0	0	0	0	0
Sh2	0	0	0	0	0	0	0	0	0	0
Sh3	0	0	0	0	0	0	0	0	0	0
Sh4	0	0	0	0	0	0	0	0	0	0
Sh5	0	0	0	0	0	0	0	0	0	0

(a), (b), (c), and (d) represent four pairs of BSP primers, respectively, which were numbered in Table 1.
(A): for 1st week MI group.
(B): for 2nd week MI group.
(C): for 3rd week MI group and SH for Sham group.