Sialic Acid Expression in the Mosquito Aedes aegypti and Its Possible Role in Dengue Virus-Vector Interactions
Table 1
Identification of DENV-2 binding proteins from Ae. aegypti SGs and saliva proteins.
Protein name
NCBI accession number
MW (kDa)
Number of matched peptides
Score
Sequence coverage (%)
Gel
Database
SG protein extract
Apyrase [Aedes aegypti]
gi∣556272
62.820
62.691
14
404
19%
Salivary serpin [Aedes aegypti]
gi∣94469320
51.617
47.765
49
712
60%
D7 protein, putative [Aedes aegypti]
gi∣157113327
37.200
38.603
46
862
44%
Long form D7Bclu1 salivary protein [Aedes aegypti]
gi∣16225992
37.200
38.579
5
86
15%
D7 protein [Aedes aegypti]
gi∣159557
37.200
37.005
3
51
9%
Putative 34 kDa secreted protein [Aedes aegypti]
gi∣18568296
34.833
36.154
18
547
33%
Putative 34 kDa family secreted salivary protein [Aedes aegypti]
gi∣94468336
34.833
35.698
20
533
33%
30 kDa salivary gland allergen Aed a 3 [Aedes aegypti]
gi∣2114497
32.628
27.130
37
479
55%
Allergen, putative [Aedes aegypti]
gi∣157133926
32.628
29.529
13
216
31%
Short form D7Cclu23 salivary protein [Aedes aegypti]
gi∣16225995
16.947
17.676
10
150
24%
Putative salivary C-type lectin [Aedes aegypti]
gi∣94468370
16.947
17.202
5
104
17%
Putative 14.5 kDa salivary protein [Aedes aegypti]
gi∣94468650
14.862
17.039
6
117
40%
Protein translocation complex beta subunit, putative [Aedes aegypti]
gi∣157138304
9.397
10.329
2
75
24%
Saliva
D7 Protein putative [Aedes aegypti]
gi∣157113327
45.23
39.173
18
178
35%
Proteins were identified by LC/ESI-MS/MS analysis after gel trypsin digestion. The table shows the protein name, the NCBI accession number, the theoretical (database) and observed (gel) MWs, the number of peptide sequences matched in the MASCOT database, the corresponding percentage sequence coverage, and the MASCOT score. The criteria used for accepting a protein as a valid identification were two or more tryptic peptide matches with the protein sequence and at least one peptide with < 0.05.
