Engineering Isoprenoid Biosynthesis in Artemisia annua L. for the Production of Taxadiene: A Key Intermediate of Taxol

Figure 3

Southern blot and semiquantitative RT-PCR analysis of transgenic A. annua. (a) Southern blot analysis of A. annua transformed with pCAMBIA1304::p35s-TXS-nos. Lane assignment: 1 λ/HindIII DNA molecular marker; 2–10 genomic DNA isolated from independent transgenic A. annua lines 3, 9, 11, 55, 56, 19, 21, 38, and 50; 11 pCAMBIA1304-TXS; 12 genomic DNA of untransformed wildtype A. annua (b) and (c) semiquantitative RT-PCR analysis for the expression of TXS in independent transgenic A. annua lines 50, 55, 11, 38, 3, 19, and 56. ck indicates the untransformed wildtype control A. annua; UBC: ubiquitin conjugation gene; TXS: taxadiene synthase gene.

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