Proteomic Changes of Tissue-Tolerable Plasma Treated Airway Epithelial Cells and Their Relation to Wound Healing
Table 1
Ranking of response mechanisms and adaptation of cellular functions due to TTP treatment as revealed by IPA. The charts were established by using Ingenuity Pathway Analyses (IPA) to relate regulated proteins to their corresponding molecular mechanism and cellular function.
(a) The “Top Tox List” displays the response mechanisms, which are most affected by several stress factors emerging due to TTP treatment. The top five response mechanisms are listed for every dose (30 s, 60 s, and 120 s) after every time point (24 h, 48 h, and 72 h). Numbers (1–5) represent the ranking of the response mechanisms for every parameter
Top Tox List
24 h
48 h
72 h
30 s
60 s
120 s
30 s
60 s
120 s
30 s
60 s
120 s
Nrf2-mediated oxidative stress
1
1
1
4
3
3
3
Decreases permeability transition of mitochondrial membrane
2
1
5
1
Mitochondrial dysfunction
3
Response to proteinuria-induced oxidative stress in renal proximal tubulus cells
4
2
Increases bradycardia
5
5
Renal necrosis/cell death
2
3
Aryl hydrocarbon receptor signaling
3
2
PPARα/RXRα activation
4
3
4
Increases transmembrane potential of mitochondrial membrane
5
5
4
5
Xenobiotic metabolism signaling
4
Mechanisms of gene regulation by peroxisome proliferators via PPARα
3
1
2
4
Increases renal proliferation
4
Oxidative stress
2
1
1
2
2
PXR/RXR activation
5
3
Cell cycle: G2/M DNA checkpoint regulation
5
1
TR/RXR activation
2
5
Acute renal failure panel
4
(b) The chart displays the most influenced molecular and cellular functions after 30 s, 60 s, and 120 s of TTP treatment and incubation time of 24 h, 48 h, and 72 h. Numbers represent the ranking of the top five cellular functions for every parameter
