Osteopontin Involves Cisplatin Resistance and Poor Prognosis in Oral Squamous Cell Carcinoma

Figure 4

Endogenous OPN depletion by OPN shRNA sensitized the cytotoxicity to cisplatin in head and neck cancer cells. (a and b) The endogenous expression level of OPN was determined by western blotting and quantitative RT-PCR in SAS cell transfected with shControl or shOPN. Data are representative of three independent experiments. (c) SAS cell transfected with shControl or shOPN were incubated with 20 μM cisplatin for 72 hours, and their viability was measured and compared to that of untreated respective cells. (d) The colony formation ability of shControl and shOPN cells treated with cisplatin (20 μM).

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