HMGB1-Induced Cross Talk between PTEN and miRs 221/222 in Thyroid Cancer

Figure 4

Expression of miR221 and miR222 after transfection with antagomiRs 221/222 and in transfectants after treatment with HMGB1. Q-RT-PCR analysis of miR221 and miR222 level expression in CAL62 and BCPAP cells transfected with miR221 and miR222 antagonists (transfectants: miR221⁻/miR222⁻) or negative control (CTR), at 25 nM final concentration for 24 h, where indicated transfectants were treated with 10 nM HMGB1 for 24 h. Data were normalized with U6b small nuclear RNA (snRNA) expression and reported as mean ± SD of 3 experiments ().