HPTLC Analysis of Bioactivity Guided Anticancer Enriched Fraction of Hydroalcoholic Extract of Picrorhiza kurroa

Table 1

HPTLC fingerprint data of mother extract and different fractionation of P. kurroa.

Extracts	Solvent system	Visualization	Number of major spots and values
Hydroalcoholic extract/mother extract	Methanol : ethyl acetate : formic acid (0.5 : 5 : 1, v/v/v)	Anisaldehyde sulphuric acid at 520 nm	(07) 0.11, 0.16, 0.25, 0.35, 0.47, 0.63, 0.96.
Hexane fraction	Toluene : ethyl acetate (1.8 : 0.1, v/v)	Anisaldehyde sulphuric acid at 520 nm	(09) 0.09, 0.13, 0.19, 0.24, 0.38, 0.48, 0.53, 0.61, 0.87
DCM fraction	Toluene : ethyl acetate : formic acid (1.5 : 0.6 : 0.1, v/v/v)	At 254 nm	(08) 0.11, 0.16, 0.24, 0.28, 0.44, 0.53, 0.66, 0.77
n-Butanol fraction	Methanol : ethyl acetate : formic acid (0.5 : 5 : 1, v/v/v)	Anisaldehyde sulphuric acid at 520 nm	(09) 0.10, 0.13, 0.18, 0.20, 0.23, 0.27, 0.32, 0.37.
Acetone fraction	Methanol : ethyl acetate : formic acid (0.5 : 5 : 1, v/v/v)	Anisaldehyde sulphuric acid at 520 nm	(07) 0.08, 0.14, 0.24, 0.34, 0.45, 0.62, 0.80.
Methanol fraction	Methanol : ethyl acetate : formic acid (0.5 : 5 : 1, v/v/v)	Anisaldehyde sulphuric acid at 520 nm	(08) 0.08, 0.13, 0.22, 0.33, 0.43, 0.60, 0.67, 0.79