HPTLC Analysis of Bioactivity Guided Anticancer Enriched Fraction of Hydroalcoholic Extract of Picrorhiza kurroa

Table 2

Linearity data of chromatographic HPTLC method for cucurbitacins B, D, and E; betulinic acid; picrosides 1 and 2; and apocynin ().

S. number	Biomarkers	Solvent system	Linearity (ng spot^−1)	Equation	Regression ± SD	Slope ± SD	Intercept ± SD	LOD (ng spot^−1)	LOQ (ng spot^−1)
1	Cucurbitacin B	(Toluene : ethyl acetate : formic acid 60 : 40 : 0.5, v/v/v)	66.7–833.3		0.995 ± 0.001	2.70 ± 0.006	340.18 ± 0.040	22.06	66.86
2	Cucurbitacin D		166.6–833.33		0.996 ± 0.001	1.89 ± 0.001	80.75 ± 0.020	54.85	166.20
3	Cucurbitacin E		166.6–833.33		0.998 ± 0.001	2.33 ± 0.006	200.76 ± 0.121	55.00	166.69
4	Betulinic acid		250–2500		0.993 ± 0.002	0.504 ± 0.004	827.64 ± 0.10	82.5	250.0
5	Picroside 1	(Toluene : ethyl acetate : methanol : formic acid 40 : 50 : 10 : 0.5, v/v/v/v)	66.7–833.33		0.994 ± 0.001	5.00 ± 0.002	565.8 ± 0.0133	21.97	66.58
6	Picroside 2		67.7–833.33		0.996 ± 0.002	3.66 ± 0.045	234.86 ± 0.303	22.28	67.53
7	Apocynin		333.33–1666.66		0.991 ± 0.001	1.87 ± 0.031	152.97 ± 1.25	110.18	333.88