HPTLC Analysis of Bioactivity Guided Anticancer Enriched Fraction of Hydroalcoholic Extract of Picrorhiza kurroa

Table 3

Precision of the method for the estimation of cucurbitacins B, D, and E; betulinic acid; picrosides 1 and 2; and apocynin ().

Conc.; ( spot^−1)	Interday precision		Intraday precision		Interanalyst precision
	Mean peak area ± SD^b	% RSD^c	Mean peak area ± SD	% RSD	Mean peak area ± SD	% RSD
Cucurbitacin B
100	541.00 ± 10.53	1.94	552.0 ± 10.81	1.95	541.0 ± 11.00	2.03
200	860.66 ± 15.04	1.74	875.0 ± 16.50	1.88	859.66 ± 16.62	1.93
400	1585.0 ± 26.72	1.68	1594.66 ± 25.71	1.61	1580.66 ± 29.36	1.85
Cucurbitacin D
200	461.33 ± 10.01	2.17	470.00 ± 10.00	2.12	442.66 ± 8.73	1.97
400	825.00 ± 15.52	1.88	837.33 ± 15.94	1.90	838.33 ± 16.04	1.91
800	1634.33 ± 32.12	1.96	1647.33 ± 33.29	2.02	1652.06 ± 34.59	2.09
Cucurbitacin E
200	637.33 ± 13.01	2.04	641.0 ± 12.66	1.97	632.66 ± 12.85	2.03
400	1157.33 ± 19.75	1.70	1172.66 ± 20.40	1.73	1162.66 ± 21.54	1.85
800	2138.0 ± 42.22	1.97	2135.66 ± 46.36	2.17	2135.33 ± 40.07	1.87
Betulinic acid
250	979.66 ± 18.50	1.88	976 ± 20.59	2.10	981.33 ± 19.55	1.99
500	1039.66 ± 21.00	2.02	1022.66 ± 19.55	1.91	1031.33 ± 20.03	1.94
1000	1374 ± 25.89	1.88	1375.66 ± 25.53	1.85	1387.33 ± 26.63	1.91
Picroside 1
100	941.00 ± 17.50	1.84	944.0 ± 19.50	2.05	943.0 ± 17.08	1.81
200	1642.66 ± 33.5	2.04	1639.33 ± 30.92	1.88	1644.33 ± 32.86	1.99
400	2936.0 ± 56.95	1.93	2940.0 ± 56.66	1.92	2929.33 ± 59.65	2.03
Picroside 2
100	502.0 ± 9.53	1.90	504.66 ± 10.50	2.08	506.0 ± 9.16	1.81
200	1005.66 ± 18.0	1.84	1008.33 ± 18.94	1.82	1007.33 ± 20.10	1.99
400	1916.66 ± 33.08	1.72	1919.33 ± 37.01	1.92	1922.66 ± 40.69	2.11
Apocynin
400	795.0 ± 15.01	1.88	799.0 ± 15.52	1.94	803.00 ± 15.09	1.88
800	1583.33 ± 31.0	1.95	1589.33 ± 32.25	2.02	1594.33 ± 31.65	1.98
1000	2225.66 ± 45.93	2.05	2231.33 ± 41.63	1.86	2227.33 ± 40.50	1.81

Nanogram. Standard deviation. Relative standard deviation.