The Use of an IL-1 Receptor Antagonist Peptide to Control Inflammation in the Treatment of Corneal Limbal Epithelial Stem Cell Deficiency

Figure 10

Immunocytochemical K3, K19, and p63 staining of primary LESC cultures cultured on (a) glass coverslips and (b) functionalised contact lenses. All corneal epithelial cells and putative LESC markers (K3, K19, and p63) were present indicating the presence of limbal epithelial cells on the functionalised contact lens. The curve of the contact lens can be seen for images in (b). The tightly packed, cobblestone morphology characteristic of epithelial cells can be observed following cytoskeletal staining with phalloidin. Cell nuclei were stained with DAPI (Leica confocal microscope, mag ×200).

(a)

(b)