Performances and Reliability of Bruker Microflex LT and VITEK MS MALDI-TOF Mass Spectrometry Systems for the Identification of Clinical Microorganisms

Table 6

Detailed results of microorganisms that were either misidentified or could not be identified by at least one MALDI-TOF system or phenotypic identification.

Correct ID	Number of isolates	On-site phenotypic identification	Secondary phenotypic identification or molecular identification  (16S or ITS1/2 sequencing)	VITEK	Bruker
Nonfermentatives
Achromobacter xylosoxidans	1	Achromobacter denitrificans	Achromobacter xylosoxidans	Achromobacter denitrificans/xylosoxidans	Achromobacter xylosoxidans
Acinetobacter baumannii	1	Cedecea lapegei	Acinetobacter baumannii	Acinetobacter baumannii complex	Acinetobacter baumannii
Acinetobacter baumannii	1	Escherichia coli	Acinetobacter baumannii complex	Acinetobacter baumannii complex	Acinetobacter baumannii
Acinetobacter baumannii	1	Klebsiella pneumoniae	Acinetobacter baumannii	Acinetobacter baumannii complex	Acinetobacter baumannii
Acinetobacter baumannii	1	Pseudomonas aeruginosa	Acinetobacter baumannii	Acinetobacter baumannii complex	Acinetobacter baumannii
Acinetobacter baumannii	1	Staphylococcus hominis	Acinetobacter baumannii complex	Acinetobacter baumannii complex	Acinetobacter baumannii
Acinetobacter baumannii	2	Acinetobacter baumannii	Acinetobacter baumannii	No identification	Acinetobacter baumannii
Acinetobacter baumannii	2	Acinetobacter baumannii	Acinetobacter baumannii	Acinetobacter baumannii complex	No identification
Acinetobacter guillouiae	1	Acinetobacter lwoffii	Acinetobacter guillouiae	Acinetobacter baumannii complex	Acinetobacter guillouiae
Acinetobacter johnsonii	1	Acinetobacter lwoffii	Acinetobacter johnsonii	Acinetobacter johnsonii	Acinetobacter johnsonii
Acinetobacter radioresistens	1	Acinetobacter lwoffii	Acinetobacter radioresistens	Acinetobacter radioresistens	Acinetobacter radioresistens
Aeromonas hydrophila	1	Aeromonas sobria	Aeromonas hydrophila	Aeromonas hydrophila/caviae	Aeromonas hydrophila
Aeromonas hydrophila	1	Aeromonas hydrophila/caviae	Aeromonas hydrophila	Aeromonas hydrophila/caviae	Aeromonas caviae
Aeromonas sobria	1	Aeromonas sobria	Aeromonas sobria	Aeromonas hydrophila/caviae, sobria	No identification
Alcaligenes faecalis	1	Chromobacterium violaceum	Alcaligenes faecalis	Alcaligenes faecalis	Alcaligenes faecalis
Burkholderia cepacia	2	Burkholderia cepacia	Burkholderia cepacia	Burkholderia cepacia/vietnamiensis	Burkholderia cenocepacia
Comamonas aquatica	1	Comamonas testosteroni	Comamonas aquatic	Delftia acidovorans	Comamonas aquatica
Delftia acidovorans	1	No identification	Delftia acidovorans	D. acidovorans	D. acidovorans
Delftia acidovorans	1	No identification	Delftia acidovorans	Delftia acidovorans	Delftia acidovorans
Pseudomonas aeruginosa	3	Acinetobacter baumannii	Pseudomonas aeruginosa	Pseudomonas aeruginosa	Pseudomonas aeruginosa
Pseudomonas aeruginosa	1	Enterobacter aerogenes	Pseudomonas aeruginosa	Pseudomonas aeruginosa	Pseudomonas aeruginosa
Pseudomonas aeruginosa	1	Pseudomonas aeruginosa	Pseudomonas aeruginosa	Porphyromonas gingivalis	Pseudomonas aeruginosa
Stenotrophomonas maltophilia	1	Pseudomonas aeruginosa	Stenotrophomonas maltophilia	Stenotrophomonas maltophilia	Stenotrophomonas maltophilia
Enterobacteriaceae
Chryseobacterium indologenes	1	No identification	Chryseobacterium indologenes	Chryseobacterium indologenes	Chryseobacterium indologenes
Citrobacter braakii	1	Citrobacter freundii	Citrobacter braakii	Citrobacter braakii/farmeri/freundii	Citrobacter braakii
Citrobacter freundii	1	No identification	Citrobacter freundii	Citrobacter freundii	Citrobacter freundii
Citrobacter sedlakii	1	Citrobacter amalonaticus	Citrobacter sedlakii	Citrobacter sedlakii	Citrobacter sedlakii
Citrobacter youngae	1	No identification	Citrobacter youngae	Citrobacter freundii/youngae	Citrobacter youngae
Citrobacter youngae	1	Citrobacter youngae	Citrobacter youngae	Citrobacter freundii/youngae	Citrobacter freundii
Enterobacter asburiae	1	Escherichia coli	Enterobacter cloacae complex	Enterobacter cloacae/asburiae	Enterobacter asburiae
Enterobacter asburiae	1	Enterobacter cloacae complex	Enterobacter asburiae	Enterobacter cloacae/asburiae	Enterobacter cloacae
Enterobacter cancerogenous	1	Enterobacter aerogenes	Enterobacter cancerogenous	Enterobacter cancerogenous	Enterobacter cancerogenous
Enterobacter cloacae	1	Enterobacter cancerogenus	Enterobacter cloacae complex	Enterobacter cloacae/asburiae	Enterobacter cloacae
Enterobacter cloacae	1	No identification	Enterobacter cloacae complex	Enterobacter cloacae/asburiae	Enterobacter cloacae
Enterobacter cloacae	1	No identification	Enterobacter cloacae	Enterobacter cloacae/asburiae	Enterobacter cloacae
Enterobacter cloacae	1	Enterobacter cloacae	Enterobacter cloacae	Enterobacter cloacae/asburiae	Enterobacter asburiae
Enterobacter cloacae	1	Enterobacter cloacae	Enterobacter cloacae	Enterobacter cloacae/asburiae	Enterobacter asburiae
Enterobacter cloacae	1	No identification	Enterobacter cloacae	Enterobacter cloacae/asburiae	Enterobacter cloacae
Klebsiella oxytoca	1	Escherichia coli	Klebsiella oxytoca	Klebsiella oxytoca	Klebsiella oxytoca
Klebsiella pneumoniae	1	Acinetobacter baumannii	Klebsiella pneumonia	Klebsiella pneumoniae	Klebsiella pneumoniae
Klebsiella pneumoniae	1	Enterobacter aerogenes	Klebsiella pneumonia	Klebsiella pneumoniae	Klebsiella pneumoniae
Klebsiella pneumoniae	1	Enterobacter aerogenes	Klebsiella pneumoniae	Klebsiella pneumoniae	Klebsiella pneumoniae
Klebsiella pneumoniae	1	Escherichia coli	Klebsiella pneumonia	Klebsiella pneumoniae	Klebsiella pneumoniae
Kluyvera cryocrescens	1	Raoultella planticola	Kluyvera cryocrescens	No identification	No identification
Proteus mirabilis	1	Stenotrophomonas maltophilia	Proteus mirabilis	Proteus mirabilis	Proteus mirabilis
Raoultella ornithinolytica	1	No identification	Raoultella ornithinolytica	Raoultella ornithinolytica/planticola	Raoultella ornithinolytica
Shigella boydii	1	Shigella boydii	Shigella boydii	Escherichia coli	Escherichia coli
Staphylococci
Staphylococcus aureus	1	Acinetobacter baumannii	Staphylococcus aureus	Staphylococcus aureus	Staphylococcus aureus
Staphylococcus hominis	1	Acinetobacter baumannii	Staphylococcus hominis	Staphylococcus hominis	Staphylococcus hominis
Staphylococcus epidermidis	1	Enterococcus faecium	Staphylococcus epidermidis	Staphylococcus epidermidis	Staphylococcus epidermidis
Staphylococcus simulans	1	Granulicatella elegans	Staphylococcus simulans	Staphylococcus haemolyticus	Staphylococcus simulans
Staphylococcus haemolyticus	1	Staphylococcus aureus	Staphylococcus haemolyticus	Staphylococcus haemolyticus	Staphylococcus haemolyticus
Staphylococcus pettenkoferi	1	Staphylococcus auricularis	Staphylococcus pettenkoferi	Staphylococcus auricularis/capitis	Staphylococcus pettenkoferi
Staphylococcus hominis	1	Staphylococcus epidermidis	Staphylococcus hominis	Staphylococcus hominis	Staphylococcus hominis
Staphylococcus aureus	1	Staphylococcus haemolyticus	Staphylococcus aureus	Staphylococcus aureus	Staphylococcus aureus
Staphylococcus capitis	1	Staphylococcus haemolyticus	Staphylococcus capitis	Staphylococcus capitis	Staphylococcus capitis
Staphylococcus aureus	1	Staphylococcus hominis	Staphylococcus aureus	Staphylococcus aureus	Staphylococcus aureus
Staphylococcus epidermidis	1	Staphylococcus hominis	Staphylococcus epidermidis	Staphylococcus epidermidis	Staphylococcus epidermidis
Staphylococcus epidermidis	1	Staphylococcus lentus	Staphylococcus epidermidis	Staphylococcus epidermidis	Staphylococcus epidermidis
Staphylococcus hominis	1	Staphylococcus saprophyticus	Staphylococcus hominis	Staphylococcus hominis	Staphylococcus hominis
Staphylococcus hominis	1	Staphylococcus warneri	Staphylococcus hominis	Staphylococcus hominis	Staphylococcus hominis
Staphylococcus hominis	2	Staphylococcus warneri	Staphylococcus hominis	Staphylococcus hominis	Staphylococcus hominis
Staphylococcus haemolyticus	1	Staphylococcus warneri	Staphylococcus haemolyticus	Staphylococcus haemolyticus	Staphylococcus haemolyticus
Staphylococcus epidermidis	1	Staphylococcus epidermidis	Staphylococcus epidermidis	Staphylococcus epidermidis	Bacillus pumilus
Staphylococcus epidermidis	1	Staphylococcus epidermidis	Staphylococcus epidermidis	Staphylococcus epidermidis	Dermobacter hominis
Staphylococcus aureus	1	No identification	Staphylococcus aureus	Staphylococcus aureus	Staphylococcus aureus
Staphylococcus lugdunensis	1	No identification	Staphylococcus lugdunensis	Staphylococcus lugdunensis	Staphylococcus lugdunensis
Staphylococcus epidermidis	1	Staphylococcus epidermidis	Staphylococcus epidermidis	Staphylococcus epidermidis	No identification
Streptococci
Leuconostoc mesenteroides	1	Staphylococcus capitis	Leuconostoc mesenteroides	No identification	Staphylococcus pettenkoferi
Streptococcus agalactiae	3	No identification	Streptococcus agalactiae	Streptococcus agalactiae	Streptococcus agalactiae
Streptococcus dysgalactiae	1	No identification	Streptococcus dysgalactiae	Streptococcus dysgalactiae	Streptococcus dysgalactiae
Streptococcus mitis	1	Streptococcus mitis	Streptococcus mitis	Streptococcus parasanguinis/mitis/oralis	Streptococcus pneumoniae
Streptococcus salivarius	1	Streptococcus lentus	Streptococcus salivarius	Streptococcus salivarius	Streptococcus salivarius
Enterococci & other Gr(+) cocci
Enterococcus faecalis	1	Escherichia coli	Enterococcus faecalis	Enterococcus faecalis	Enterococcus faecalis
Enterococcus faecium	1	Enterococcus faecalis	Enterococcus faecium	Enterococcus faecium	Enterococcus faecium
Enterococcus faecalis	2	Enterococcus faecium	Enterococcus faecalis	Enterococcus faecalis	Enterococcus faecalis
Enterococcus gallinarum	1	Enterococcus faecium	Enterococcus gallinarum	Enterococcus gallinarum	Enterococcus gallinarum
Enterococcus faecium	2	Enterococcus gallinarum	Enterococcus faecium	Enterococcus faecium	Enterococcus faecium
Enterococcus faecalis	1	Serratia fonticola	Enterococcus faecalis	Enterococcus faecalis	Enterococcus faecalis
Enterococcus faecalis	1	Staphylococcus hominis	Enterococcus faecalis	Enterococcus faecalis	Enterococcus faecalis
Coryneforms & other Gr(+) bacilli
Corynebacterium striatum	1	Actinomyces spp.	Corynebacterium striatum	Corynebacterium striatum	Corynebacterium striatum
Corynebacterium urealyticum	1	Brucella spp.	Corynebacterium urealyticum	Corynebacterium urealyticum	Corynebacterium urealyticum
Corynebacterium appendicis	1	Corynebacterium jeikeium	Corynebacterium appendicis	Corynebacterium pseudodiphtericum	Corynebacterium pseudodiphtericum
Corynebacterium ureicelerivorans	1	Corynebacterium jeikeium	Corynebacterium ureicelerivorans	Corynebacterium afermentans	Corynebacterium afermentans
Corynebacterium mucifaciens	2	Corynebacterium jeikeium	Corynebacterium mucifaciens	No identification	Corynebacterium mucifaciens
Corynebacterium ureicelerivorans	1	Corynebacterium jeikeium	Corynebacterium ureicelerivorans	No identification	Corynebacterium coyleae
Corynebacterium glaucum	1	Corynebacterium jeikeium	Corynebacterium glaucum	No identification	No identification
Corynebacterium mucifaciens	1	Clostridium spp.	Corynebacterium mucifaciens	Rhizobium radiobacter	Corynebacterium mucifaciens
Corynebacterium coyleae	1	Dermococcus/Kytococcus/; Micrococcus	Corynebacterium coyleae	Arthrobacter cumminsii	Corynebacterium coyleae
Lactobacillus paracasei	1	Pediococcus pentosaceus	Lactobacillus paracasei	Lactobacillus casei/paracasei	Lactobacillus paracasei
Corynebacterium striatum	1	Propionibacterium acnes	Corynebacterium striatum	Corynebacterium striatum	Corynebacterium striatum
Bacillus simplex	1	Sphingomonas paucimobilis	Bacillus simplex	Bacillus simplex	Bacillus simplex
Corynebacterium amycolatum	1	Bacillus spp.	Corynebacterium amycolatum	Corynebacterium amycolatum/xerosis	Corynebacterium amycolatum
Corynebacterium jeikeium	2	Bacillus spp.	Corynebacterium jeikeium	Corynebacterium jeikeium	Corynebacterium jeikeium
Corynebacterium striatum	2	Bacillus spp.	Corynebacterium striatum	Corynebacterium striatum	Corynebacterium striatum
Actinomyces naeslundii	1	Actinomyces naeslundii	Actinomyces naeslundii	No identification	Actinomyces spp.
HACEK & other Gr(−) cocci
Moraxella catarrhalis	1	Arcanobacterium hemoliticum	Moraxella catarrhalis	Moraxella catarrhalis	Moraxella catarrhalis
Haemophilus influenzae	1	Bacillus spp.	Haemophilus influenzae	Haemophilus influenzae	Haemophilus influenzae
Pasteurella multocida	1	Bacillus spp.	Pasteurella multocida	Pasteurella multocida	Pasteurella multocida
Others
Bergeyella zoohelcum	1	No identification	Bergeyella zoohelcum	Bergeyella zoohelcum	Bergeyella zoohelcum
Wautersiella falsenii	1	Weeksella virosa	Wautersiella falsenii	No identification	No identification
Myroides odoratimimus	1	Myroides spp.	Myroides odoratimimus	Myroides odoratimimus	Myroides spp.
Fungi
Candida glabrata	2	Candida albicans	Candida glabrata	Candida glabrata	Candida glabrata
Candida albicans	1	Candida famata	Candida albicans	Candida albicans	Candida albicans
Candida tropicalis	1	Candida famata	Candida tropicalis	Candida tropicalis/glabrata	Candida tropicalis
Candida tropicalis	1	Candida famata	Candida tropicalis	Candida tropicalis	Candida tropicalis
Candida albicans	2	Candida glabrata	Candida albicans	Candida albicans	Candida albicans
Candida glabrata	1	Candida lusitaniae	Candida glabrata	Candida glabrata	Candida glabrata
Candida tropicalis	2	Candida parapsilosis	Candida tropicalis	Candida tropicalis	Candida tropicalis
Candida glabrata	1	Candida rugose	Candida glabrata	Candida glabrata	Candida glabrata
Candida kefyr	1	Candida sphaerica	Candida kefyr	Candida kefyr	Candida kefyr
Candida orthopsilosis	1	Candida tropicalis	Candida orthopsilosis	Candida parapsilosis	Candida orthopsilosis
Candida parapsilosis	2	Candida spp.	Candida parapsilosis	Candida parapsilosis	Candida parapsilosis
Candida orthopsilosis	1	Candida spp.	Candida orthopsilosis	Candida parapsilosis	Candida orthopsilosis
Meyerozyma caribbica	1	Candida spp.	Meyerozyma caribbica	Candida guilliermondii	Candida guilliermondii
Candida albicans	1	Stephanoascus ciferrii	Candida albicans	Candida albicans	Candida albicans
Candida krusei	1	No identification	Candida krusei	Candida krusei	Candida krusei
Candida albicans	1	Candida albicans	Candida albicans	Candida albicans	Candida tropicalis
Candida albicans	1	Candida albicans	Candida albicans	Candida albicans	Candida glabrata
Candida parapsilosis	1	Candida parapsilosis	Candida parapsilosis	Candida parapsilosis	Candida orthopsilosis
Aspergillus brasiliensis	1	Aspergillus brasiliensis	Aspergillus brasiliensis	No identification	Aspergillus brasiliensis

Molecular identification (16S or ITS1/2 sequencing).