Nampt/PBEF/Visfatin Upregulation in Colorectal Tumors, Mirrored in Normal Tissue and Whole Blood of Colorectal Cancer Patients, Is Associated with Metastasis, Hypoxia, IL1β, and Anemia

Table 1

Relationship between Nampt/PBEF/visfatin expression in colorectal tissue and clinicopathological features of CRC.

Parameter		Nampt/PBEF/visfatin		Ratio T/N
		Tumor tissue	Nontumor tissue
Sex distribution, F/M ratio		17/35
Age, median (range)		68 yrs (33–91)
Disease stage:
I	10	0.973 (0.74–1.27)^e	0.475 (0.32–0.72)^c,d,e	2.1 (1.4–3.1)^f
II	20	1.270 (0.83–1.95)^a	0.903 (0.63–1.29)^b,d,e	1.4 (1-2)^f
III	16	1.133 (0.85–1.52)^a,e	0.743 (0.52–1.06)^b,c,e	1.5 (1.3–1.9)^f
IV	5	3.074 (0.95–9.90)^a,b,d	1.615 (0.65–3.99)^b,c,d	1.9 (0.8–4.6)
Local advancement (T):
T2	13	1.217 (0.88–1.69)^a	0.565 (0.37–0.85)	2.2 (1.6–3.1)^f
T3	21	1.226 (0.83–1.82)^a	0.874 (0.61–1.25)	1.4 (1–1.9)^f
T4	17	1.365 (0.88–2.11)^a	0.901 (0.62–1.30)	1.5 (1.2–1.9)^f
Lymph node metastases (N):
N0	33	1.259 (0.94–1.69)	0.775 (0.59–1.02)	1.6 (1.3–2.1)^f
N1	11	0.983 (0.69–1.41)^g	0.627 (0.40–0.98)^g	1.6 (1.3–2.1)^f
N2	7	1.963 (1.04–3.72)^h	1.308 (0.76–2.25)^h	1.5 (1–2.3)^f
Distant metastases (M):
M0	46	1.152 (0.93–1.42)^i	0.734 (0.59–0.91)^i	1.6 (1.3–1.9)^f
M1	5	3.074 (0.95–9.90)^j	1.615 (0.65–3.99)^j	1.9 (0.8–4.6)
Anemia:
Nonanemic	20	1.050 (0.84–1.31)^k,l	0.639 (0.48–0.85)^k,l	1.6 (1.3–2.1)^f
Mild anemia	26	1.637 (1.15–2.33)^l,m	1.039 (0.76–1.43)^l,m	1.6 (1.2–2)^f
Moderate/severe	5	0.716 (0.26–2.01)^k,m	0.475 (0.20–1.15)^k,m	1.5 (0.6–3.9)
Tumor location:
Left	15	1.778 (1.14–2.77)	0.772 (0.46–1.28)	2.2 (1.5–3.3)^f
Right	18	1.135 (0.76–1.70)	0.945 (0.66–1.35)	1.2 (0.9–1.6)
Rectum	18	1.070 (0.77–1.49)	0.666 (0.51–0.88)	1.6 (1.4–1.9)^f

Data presented as means with 95% CI of normalized (against geometric mean of GAPDH and PPIA expression) relative (referred to as mean expression across sample set investigated) quantities of Nampt/PBEF/visfatin, calculated by qBasePLUS software. Information in superscript indicates significant () between-group differences calculated using one-way ANOVA and -test for independent samples. Ratio of tumor-to-normal (T/N) expression for each parameter was calculated using -test for paired samples. Data presented as mean upregulation with 95% confidence interval.
^aDifferent from normal tissue; ^bdifferent from stage I; ^cdifferent from stage II; ^ddifferent from stage III, ^edifferent from stage IV; ^fsignificantly upregulated in tumor tissues compared to matched nontumor ones (-test for paired samples); ^gdifferent from N2 cancers; ^hdifferent from N1 cancers; ⁱdifferent from M1 cancers; ^jdifferent from M0 cancers; ^kdifferent from mild anemia; ^ldifferent from moderate/severe anemia; ^mdifferent from nonanemic patients.