Antibody-Based Assays for Phenotyping of Extracellular Vesicles

Figure 3

Schematic view of the EV Array. A basis for the procedure is epoxy-silane coated slides printed with 21 identical antibody microarray prints (for 20 samples and one control). Each antibody is printed in triplicate. EV-containing samples are applied and the EVs are captured onto the slides depending on the presence of surface antigens. Detection is performed by addition of biotin-labeled detection antibodies against the exosomal markers CD9, CD63, and CD81 followed by fluorescently labeled streptavidin. A fluorescence scanner is used to get a high resolution picture from which the fluorescent signals for each antibody spot can be quantified.