Antibody-Based Assays for Phenotyping of Extracellular Vesicles

Figure 6

Schematic view of the micro-NMR technology. Three samples can be applied onto the prototype of the microfluidic cell together with buffer and antibodies. First step in the procedure is a filtration through pores of 50–150 nm after which EV surface antigens are targeted with antibodies labeled with trans-cyclooctene (TCO). For detection 1,2,4,5-tetrazine (TZ) labeled magnetic nanoparticles (MNP) are added and captured EVs are detected with the miniaturized μNMR system. The relaxation is equivalent to the amount of MNPs and thereby the amount of surface antigen present in the sample.