MALDI-TOF MS and CD Spectral Analysis for Identification and Structure Prediction of a Purified, Novel, Organic Solvent Stable, Fibrinolytic Metalloprotease from Bacillus cereus B80

Table 2

The peptide matching (12/16 matches) for / data of metalloprotease with zinc metalloprotease from Bacillus cereus (gi∣507041200∣ref∣WP_016112858.1∣).

Spectrum	Prec.	MH+	Modifications	Missed cleavages	Position	Peptide
	/	Matched
1.F2.2.1.5	2704.8679	2761.3123	MSO	0	1–23	MIHTYLGETINFHINCKKKKSVR (I)
1.F2.2.1.7	2383.300	2446.1576	CYS_CAM MSO	0	187–206	(R) VIDYVVVHEMCHMVHLNHDR (S)
1.F2.2.1.9	1638.686	1617.8601	MSO	1	39–51	(K) GTPVEYVLQLLEEK (W)
1.F2.2.1.15	1052.532	979.1244	CYS_CAM MSO	0	129–135	(R) FYYQQCK (A)
1.F2.2.1.12	1350.614	1314.5724		0	61–73	(K) EMKRVLGPQEK (V)
1.F2.2.1.13	1267.53	1260.4618	MSO	0	52–61	(K) WDWIQKTRK (E)
1.F2.2.1.16	868.4659	887.0877	MSO	0	136–143	(K) ALVEKSIK (S)
1.F2.2.1.3	1803.738	1786.1747	CYS_CAM MSO	0	172–186	(K) LQLTFNWKLAMAPPR (V)
1.F2.2.1.4	1707.594	1695.8900		0	24–38	(R) IYIDSYGNVEVQAPK (G)
1.F2.2.1.2	1765.5551	1740.1028		0	204–216	(R) SFWRLVGKIMPDYK (I)
1.F2.2.1.8	1838.717	1757.0606		0	110–123	(K) LHIYVKELEDEKIK (Q)
1.F2.2.1.1	1488.676	1494.6884	MSO	1	221–231	(K) EMEDWLALSSWK (M)