MALDI-TOF MS and CD Spectral Analysis for Identification and Structure Prediction of a Purified, Novel, Organic Solvent Stable, Fibrinolytic Metalloprotease from Bacillus cereus B80

Table 3

Effects of organic solvents on the stability of the enzyme.

Solvents	value	Day 1	Day 2	Day 4	Day 6	Day 8	Day 10
None		100	100	98.18	98	96	92
Hexane	3.98	99.26	81.37	96.71	51.29	40.21	38.98
Ethyl benzene	3.15	172.79	139.95	120.32	97.43	85.15	81.48
Xylene	3.15	106.37	182.84	176.40	160.28	132.17	118.39
Toluene	2.73	115.69	189.22	169.01	143.20	126.54	115.94
Benzene	2.13	99.95	95.58	96.95	87.22	86.54	83.1
Chloroform	2	107.6	107.84	84.457	25.94	24.52	18.93
Amyl alcohol	1.2	77.21	180.88	107.00	104.13	94.67	78.67
Butanol	0.88	94.12	154.41	125.35	104.13	14.04	8.41
Propanol 2	0.074	99.50	159.8	74.89	51.29	32.66	28.44
Acetone	−0.21	90.93	110.05	98.42	96.68	91.49	51.72
Ethanol	−0.235	85.29	100.24	88.62	61.53	50.07	38.98
Acetonitrile	−0.394	94.85	190.2	96.71	84.23	54.72	35.74
DMSO	−1.378	106.62	118.63	115.82	80.84	69.77	52.49
Glycerol	−1.93	147.30	95.83	93.77	85.11	76.54	21.58