BioMed Research International / 2015 / Article / Tab 3

Research Article

MALDI-TOF MS and CD Spectral Analysis for Identification and Structure Prediction of a Purified, Novel, Organic Solvent Stable, Fibrinolytic Metalloprotease from Bacillus cereus B80

Table 3

Effects of organic solvents on the stability of the enzyme.

Solvents valueDay 1Day 2Day 4Day 6Day 8Day 10

None10010098.18989692
Hexane3.9899.2681.3796.7151.2940.2138.98
Ethyl benzene3.15172.79139.95120.3297.4385.1581.48
Xylene3.15106.37182.84176.40160.28132.17118.39
Toluene2.73115.69189.22169.01143.20126.54115.94
Benzene2.1399.9595.5896.9587.2286.5483.1
Chloroform2107.6107.8484.45725.9424.5218.93
Amyl alcohol1.277.21180.88107.00104.1394.6778.67
Butanol0.8894.12154.41125.35104.1314.048.41
Propanol 20.07499.50159.874.8951.2932.6628.44
Acetone−0.2190.93110.0598.4296.6891.4951.72
Ethanol−0.23585.29100.2488.6261.5350.0738.98
Acetonitrile−0.39494.85190.296.7184.2354.7235.74
DMSO−1.378106.62118.63115.8280.8469.7752.49
Glycerol−1.93147.3095.8393.7785.1176.5421.58

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