Effect of Fe₃O₄ Nanoparticles on Skin Tumor Cells and Dermal Fibroblasts

Figure 2

Effect of Fe₃O₄ nanoparticles on myofibroblast formation. (a) Gene expression of αSMA was analyzed by real-time RT-PCR with normalization against HPRT1. The data represent means ± s.e.m. . CM: conditioned medium. versus TGF1 treatment only (Student’s -test). (b) Fibroblasts were grown to subconfluence. These cells were either untreated or incubated with 50 μM or 350 μM Fe₃O₄ nanoparticles for 24 h before treatment with rTGFβ1. TGFβ1 and the Fe₃O₄ nanoparticles were present for 48 h. α-tubulin served as loading control.. CM: conditioned medium.

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