100 g/L glucose plus 100 g/L D-gluconic acid. 100 g/L glucose plus 50 g/L D-gluconic acid. After initial sugars of 20 g/L xylose and 20 g/L glucose were consumed completely, a sugar mixture of 200 g/L xylose and 50 g/L glucose was fed stepwise into a bioreactor. Initial glucose 200 g/L, starting time 24 h, dilution rates 0.006/h, and influent glucose concentration 200 g/L.