Statistical Optimization of Conditions for Decolorization of Synthetic Dyes by Cordyceps militaris MTCC 3936 Using RSM

Table 3

ANOVA analysis of CCRD models generated for various dyes.

Sr. number	Source	Sum of squares	Df	Mean square	value	value, prob >
Reactive yellow 18
1	Model	15815.32	9	1757.26	4.21	0.0175 significant
2	: dye conc.	3213.33	1	3213.33	7.70	0.0196
3	: pH	3123.77	1	3123.77	7.49	0.0210
4	: incubation	995.52	1	995.52	2.39	0.1535 (nonsignificant)
5		1079.27	1	1079.27	2.59	0.1389
6		90.32	1	90.32	0.22	0.6518
7		40.50	1	40.50	0.097	0.7618
8		14.52	1	14.52	0.0151	0.8267
9		1986.89	1	1986.09	6.91	0.0252
10		482.85	1	482.85	1.16	0.3074
11	Residual	4173.36	10	417.34
12	Lack of fit	4173.36	5	834.61
13	Pure error	0.000	5	0.000
14	Cor. total	19988.67	19
0.8043, adequate precision 7.894
Reactive red 31
1	Model	6933.16	9	770.35	2.31	0.1041 not significant
2	: dye conc.	1046.11	1	1046.11	3.14	0.1069
3	: pH	1256.93	1	1256.93	3.77	0.0809
4	: incubation	1174.09	1	1174.09	3.52	0.0901
5		206.45	1	206.45	0.62	0.4496
6		3.30	1	3.30	9.904E − 003	0.9227
7		25.42	1	25.42	0.076	0.7881
8		427.00	1	427.00	1.28	0.2842
9		2527.02	1	2527.02	7.58	0.0204
10		64.29	1	64.29	0.19	0.6699
11	Residual	3334.55	10	333.45
12	Lack of fit	3327.71	5	665.54	486.70	<0.0001
13	Pure error	6.84	5	1.37	2.31	0.1041
14	Cor. total	10267.71	19
0.6752, adequate precision 6.482
Reactive red 74
1	Model	6876.62	9	764.07	3.06	0.0483 significant
2	: dye conc.	282.71	1	282.71	1.13	0.3127
3	: pH	643.52	1	643.52	2.57	0.1398
4	: incubation	1612.56	1	1612.56	6.45	0.0294
5		19.84	1	19.84	0.079	0.7839
6		116.28	1	116.28	0.46	0.5108
7		33.78	1	33.78	0.14	0.7209
8		150.73	1	150.73	0.60	0.4555
9		3845.40	1	3845.40	15.38	0.0029
10		549.00	1	549.00	2.20	0.1692
11	Residual	2500.81	10	250.08
12	Lack of fit	2473.93	5	494.79	92.03	<0.0001
13	Pure error	26.88	5	5.38
14	Cor. total	9377.42	19
0.6752, adequate precision 5.239
Reactive green 19
1	Model	9189.45	9	1021.05	4.52	0.0137 significant
2	: dye conc.	873.91	1	873.91	3.87	0.0775
3	: pH	1782.22	1	1782.22	7.89	0.0185
4	: incubation	1614.99	1	1614.99	7.15	0.0233
5		36.68	1	36.68	0.16	0.6954
6		6.53	1	6.53	0.029	0.8683
7		22.88	1	22.88	0.10	0.7568
8		291.80	1	291.80	1.29	0.2822
9		4276.32	1	4276.32	18.94	0.0014
10		827.47	1	827.47	3.66	0.0846
11	Residual	2258.35	10	225.83
12	Lack of fit	2225.27	5	445.05	67.27	0.0001
13	Pure error	33.08	5	6.62
14	Cor. total	11447.80	19
0.8027, adequate precision 6.461
Reactive black 8
1	Model	12726.34	9	1414.04	3.44	0.0337 significant
2	: dye conc.	1426.78	1	1426.78	3.47	0.0920
3	: pH	1408.20	1	1408.20	3.43	0.0938
4	: incubation	1811.25	1	1811.25	4.41	0.0621
5		165.53	1	165.53	0.40	0.5398
6		31.40	1	31.40	0.076	0.7878
7		12.78	1	12.78	0.031	0.8635
8		52.88	1	52.88	0.13	0.7272
9		7153.88	1	7153.88	17.41	0.0019
10		1211.01	1	1211.01	2.95	0.1167
11	Residual	4108.02	10	410.80
12	Lack of fit	4092.24	5	818.45	259.38	<0.0001
13	Pure error	15.78	5	3.16
14	Cor. total	16834.36	19
0.7560, adequate precision 6.410