Research Article
Identification of Type II Interferon Receptors in Geese: Gene Structure, Phylogenetic Analysis, and Expression Patterns
Table 1
List of primers and sequences.
| Methods | Gene name | Primer name | Nucleotide sequence (5′-3′) |
| Reverse transcription | | Oligo(dT)18 | TTTTTTTTTTTTTTTTTT |
| Partial sequence | IFNGR1 | F1 | TTAAAGCTGTTGTTGGATCA | R1 | CAATCACASGYTGTTCTTC | IFNGR2 | F2 | CTGAGGTGGTCTCCTGTTA | R2 | TCAAATACTCTTCAAWGTGTG |
| 3RACE | | AP | CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGC (T)18 | AP1 | CCAGTGAGCAGAGTGACG | AP2 | GAGGACTCGAGCTCAAGC |
| 5RACE | | AAP | GGCCACGCGTCGACTACGGGIIGGGIIGGGIIGGGIIG | AUAP | GGCCACGCGTCGACTAGTAC |
| 3RACE-GSP | IFNGR1 | G1-3GSP1 | GGCACCAGACAAAGTGGAAGAGTC | G1-3GSP2 | TGCAGAAGATTACAGAGGAGGTCC | IFNGR2 | G2-3GSP1 | TGGACTGCGGAGAATCCCGGAATG | G2-3GSP2 | CAATGAGTGAGACAACCAGAGCTG |
| 5RACE-GSP | IFNGR1 | G1-5GSP1 | ATCCCAAAAAGTCACC | G1-5GSP2 | GAAATACAGGATGGTAAATATCAAC | G1-5GSP3 | GAGAGATCCAGTTTTGGAGGTC | IFNGR2 | G2-5GSP1 | CATTCTCCCAGTAG | G2-5GSP2 | AGTCACGCTGTTCACTTTAGGG | G2-5GSP3 | ATTCCACCCAGTCAGAAGTCAT |
| Real-time PCR | IFNGR1 | goqRT-G1-F | GCATTCAGGTTCCTCTTG | goqRT-G1-R | AAGCGTTATCCATGTTCAG | IFNGR2 | goqRT-G2-F | AATCTTCTCCACGTTTACCG | goqRT-G2-R | CAGTAGAAGTAATTCATGGTG | β-actin | goqRT-βactin-F | TCCCTGGAGAAGAGCTACGA | goqRT-βactin-R | GTGTTGGCGTACAGGTCCTT |
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Degenerate bases: Y = C + T; W = A + T; and S = C + G.
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